• Dessy Triana Department of Parasitology, Faculty of Medicine and Health Sciences Universitas Bengkulu, Indonesia
  • Fitaloca N Siregar Undergraduate Program of Medicine, Faculty of Medicine and Health Sciences Universitas Bengkulu, Indonesia
  • Eka Purnama Wulan Undergraduate Program of Medicine, Faculty of Medicine and Health Sciences Universitas Bengkulu, Indonesia
  • Tatik Suteky Department of Farming, Faculty of Agriculture Universitas Bengkulu, Indonesia
  • Swandito Wicaksono Department of Physiology, Faculty of Medicine and Health Sciences Universitas Bengkulu, Indonesia
Keywords: Dengue, Aedes aegypti, Aedes albopictus, Entomological Parameter, Insecticide Resistance


In Bengkulu, as in other areas of Indonesia, dengue is one of the most important vector-borne diseases. The primary strategy to control dengue is by reducing the vector population using insecticides. However, applications of insecticides becomes a contributing factor in the development of vector resistance. As there is no effective dengue drug or safe vaccine available, vector control remains the most effective intervention to reduce incidence and prevent transmission and outbreak. This study aimed to determine the entomological parameters and resistance status to temephos of Aedes aegypti larvae from Lingkar Barat village and Jalan Gedang village (dengue endemic areas). This study used cross-sectional and experimental with post-control only design. The sampling refers to the guidelines for dengue entomology surveys according to WHO 1999 criteria. Aedes aegypti eggs were collected by ovitraps and reared to larvae stage. Susceptibility test of larvae to the temephos was estimated using standard WHO. Mortality of larvae Ae. aegypti after 24 hours with multiple concentration of temephos indicative of highly resistant.  Entomological parameters for house index (HI), container index (CI) and container pupa index (CPI) in Jalan Gedang village and Lingkar Barat village were estimated: 7%, 12% for HI and 1.97%, 3.43% for CI, and 5%, 2% for CPI, respectively. The proportion of controllable sites is higher than disposable sites, it has a high risk as breeding places for mosquitoes indicates a high potential risk as breeding place and transmission of dengue. Awareness sanitation in the home and surrounding environment must be possessed by each individual community in order to prevent and overcome the incidence of dengue. These data should be of value in formulating dengue control programs.


Gubler D. Epidemic dengue/dengue hemorrhagic fever as a public health, social and economic problem in the 21st century. Trends Microbiol. 2002;10:100–3.

WHO. Global Strategy for Dengue Prevention and Control 2012–2020. WHO. 2012. 1–34 p.

Ministry of Health of Indonesia. INFODATIN (Situasi Demam Berdarah Dengue di Indonesia). Jakarta Selatan; 2016.

WHO. Comprehensive Guidelines for Prevention and Control of Dengue and Dengue Haemorrhagic Fever. WHO Regional Publication SEARO. 2011. 1–195 p.

WHO. Dengue Guidelines for Diagnosis, Treatment, Prevention and Control. WHO Library Cataloguing in Publication Data Dengue. 2009. 1–147 p.

Ministry of Health of Indonesia. Riset Kesehatan Dasar 2013. 2013.

Arslan A, Rathor HR, Mukhtar MU, Mushtaq S, Bhatti A, Asif M, et al. Spatial Distribution and Insecticide Susceptibility Status of Aedes aegypti and Aedes albopictus In Dengue Affected Urban Areas of Rawalpindi, Pakistan. J Vector Borne Dis. 2016;53(2):136–43.

Kamgang B, Marcombe S, Chandre F, Nchoutpouen E, Nwane P, Etang J, et al. Insecticide Susceptibility of Aedes aegypti and Aedes albopictus in Central Africa. Parasit Vectors 2011;4(1):79–86.

Sukowati S. Penggunaan Metode Survei Pupa Untuk Memprediksi Risiko Penularan Demam Berdarah Dengue Di Lima Wilayah Endemis Di Dki Jakarta. Media Heal Res Dev. 2013;23(1):31–40.

Mardihusodo SJ, Satoto TBT, Mulyaningsih B, Umniyati SR, Ernaningsih. Pupal Demographic and Adult Aspiration Survey of Residental and Public Sites in Yogyakarta, Indonesia to Inform Development of Targeted Sources Control Strategy for Dengue. Dengue Bull. 2011;35:141–51.

Arunachalam N, Tana S, Espino F, Kittayapong P, Abeyewickreme W, Whai KT, et al. Eco-Bio-Social Determinans of Dengue Vector Breeding; A Multicountry Study In Urban and Periurban Asia. Bull World Heal Org. 2010;88:173–84.

Selvan S, Jebanesan A, Reetha D. Entomofaunal diversity of tree hole mosquitoes in Western and Eastern Ghats hill ranges of Tamilnadu, India. Acta Trop. 2016 Jul 1;159:69–82.

Mullen GR (Gary R, Durden LA. Medical and veterinary entomology. London: Elsevier; 2009. 627 p.

Selvan S, Jebanesan A. Survey and Epidemiology of Tree Hole Breeding Mosquitoes In Annamalai, University Campus, Tamilnadu, India. Int J Curr Res. 2014;6(5):6462–5.

Ministry of Health of Indonesia. Profil Kesehatan Indonesia Tahun 2017. Ministry of Health of Indonesia. Jakarta; 2018. 107–108 p

WHO. World Health Organization, Regional Office for South-East Asia. Comprehensive Guideline for Prevention and Control of Dengue and Dengue Haemorrhagic Fever. Revised and expanded edition. WHO Regional Office for South-East Asia. 2011.

WHO. Global Strategic Framework for Integrated Vector Management. World Health Organization. Geneva; 2004.

Bhat MA, Krishnamoorthy K. Original Research Article Entomological investigation and distribution of Aedes mosquitoes in. Int J Curr Microbiol Appl Sci. 2014;3(10):253–60.

Abbott WS. A Method of Computing The Effectiveness of an Insecticide. JAMCA. 1987;3(2):302–3.

WHO. Monitoring and Managing Insecticide Resistance in Aedes Mosquito Populations: Interim Guidance for Entomologist. Geneva; 2016.

BMKG. Data Online - Pusat Database - BMKG [Internet]. Badan Meteorologi, Klimatologi dan Geofisika (BMKG) Stasiun Meteorologi Fatmawati Soekarno. 2020 [cited 2020 Jul 19]. p. 1–2. Available from: http://dataonline.bmkg.go.id/home

Minhas S, Sekhon H. Entomological Survey for Dengue Vector in an Institutional Campus to Determine Whether Potential of Dengue Outbreak Exixts. Int J Med Appl Sci. 2013;2(4):164–71.

Fuadzy H, Hendri J. Indeks Entomologi dan Kerentanan Larva Aedes aegypti Terhadap Temefos di Kelurahan Karsamenak Kecamatan Kawalu Kota Tasikmalaya. Vektora J Vektor dan Reserv Penyakit. 2015;7(2):57–64.

Istiana, Heriyani F, Isnaini. Resistance Status of Aedes aegypti Larvae to Temephos in West Banjarmasin. J Epidemiol dan Penyakit Bersumber Binatang. 2012;4(2):53–8.

Prasetyowati H, Hendri J, Wahono T. Status Resistensi Aedes aegypti (Linn.) terhadap Organofosfat di Tiga Kotamadya DKI Jakarta. Balaba J Litbang Pengendali Penyakit Bersumber Binatang Banjarnegara. 2016;12(1): 1-8.

Triana D, Umniyati SR, Mulyaningsih B. Resistance status of aedes aegypti to malathion and cypermethrin in Bengkulu city, Indonesia. Southeast Asian J Trop Med Public Health. 2019;50(3):461–8.

Simbawara L. Status Resistensi Aedes aegypti Terhadap Malation Dan Temefos Serta Distribusi Spasialnya Di Daerah Endemis DBD Kabupaten Sumbawa Lalu Simbawara. [Thesis]. Sekolah Pascasarjana Institut Pertanian Bogor. 2017.

Georghiou GP, Mellon RB. Pesticide Resistance in Time and Space. In: Pest Resistance to Pesticides. New York: Plenum Press; 1983.

Hemingway J, Hawkes NJ, Mccarroll L, Ranson H. The Molecular Basis of Insecticide Resistance in Mosquitoes. Insect Biochem Mol Biol. 2004;34:653–65.

Ming LS, Adnan TH, Huck OC, Ibrahim M, Husin D, Abdullah NA, et al. Adult Aedes Mosquito And Dengue Virus Surveillance In Residential And Public Areas Of Selangor, Malaysia. Southeast Asean J Trop Med Public Heal. 2018;49(4):553–65.

Sambuaga JVI. Status Entomologi Vektor Demam Berdarah Dengue di Kelurahan Perkamil Kecamatan Tikala Kota Manado Tahun 2011. J Kesehat Lingkung. 2011;1(1):54–61.

Vijayakumar K, Sudheesh Kumar TK, Nujum ZT, Umarul F, Kuriakose A. A study on container breeding mosquitoes with special reference to Aedes (Stegomyia) aegypti and Aedes albopictus in Thiruvananthapuram district, India. J Vector Borne Dis., 2014;51(1):27–32.

Sivagnaname N, Gunasekaran K. Need for an efficient adult trap for the surveillance of dengue vectors. Indian J Med Res. 2012;136(5):739–49.

How to Cite
Triana, D., Siregar, F. N., Tri Utami, E. P. W., Suteky , T., & Wicaksono, S. (2021). ENTOMOLOGICAL PARAMETERS AND CHARACTERIZATION OF INSECTICIDE RESISTANCE IN DENGUE VECTOR AEDES AEGYPTI LARVAE FROM BENGKULU CITY, INDONESIA. Malaysian Journal of Public Health Medicine, 21(1), 96-102. https://doi.org/10.37268/mjphm/vol.21/no.1/art.467